Question 1

Which of the following instruments does not rely on impedance to enumerate cells:
A)Advia 120
B)XE-2100
C)Sapphire
D)Coulter

Accepted Answer

A

Question 2

What cellular characteristic is evaluated by radio frequency?
A)Size
B)Density
C)Shape
D)Complexity

Accepted Answer

Radio frequency is used to evaluate cellular density, specifically for applications such as determining tumor size or density. It is not typically used for evaluating cellular size, shape, or complexity.

Question 3

An analyzer is measuring a clot forming by the interaction of a stationary probe with a moving probe in a continuous motion.Suddenly the moving probe stops,and a clot is seen.Name the analyzer.
A)Diagnostica Stago
B)MDA 180
C)Trinity Biotech AMAX Destiny Plus
D)BBL Fibrometer

Accepted Answer

The BBL Fibrometer measures clot formation by the interaction of a stationary probe and a moving probe in continuous motion. The sudden stop of the moving probe indicates clot formation.

Question 4

Regarding the CELL-DYN Sapphire,which parameter is determined by the impedance principle?
A)Hemoglobin
B)Nucleated erythrocyte count
C)Platelet count
D)Total leukocyte count

Accepted Answer

The impedance principle is used to determine the platelet count in the CELL-DYN Sapphire instrument. Impedance technology measures the changes in resistance to an electrical current caused by the passage of cells through a small aperture. Since platelets are smaller than other blood cells, they produce a unique electrical signal that can be detected and counted.

Question 5

Which of the following binds to residual RNA in reticulocytes on the Sysmex XE-2100 analyzer?
A)New methylene blue
B)Propidium iodide
C)Polymethine
D)Oxazine

Accepted Answer

The answer of Which of the following binds to residual...

Question 6

Which of the following instruments uses both electromechanical and optical density as a means of clot detection?
A)STArt 4 Coagulation system
B)BBL Fibrosystem
C)Trinity Biotech Coag-A-Mate XM
D)AMAX Destiny Plus

Accepted Answer

The answer of Which of the following instruments uses both...

Question 7

Which of the following represents the degree of light scatter that reflects cell complexity on the CELL-DYN Sapphire?
A)0 degrees
B)7 degrees
C)90 degrees
D)90 degrees depolarized

Accepted Answer

The answer of Which of the following represents the degree...

Question 8

Which of the following analyzers measures reticulocytes by sphering the blood cells prior to analysis?
A)Coulter LH series
B)Coulter Gen-S
C)Abbott Sapphire
D)Sysmex XE-2100

Accepted Answer

The answer of Which of the following analyzers measures reticulocytes...

Question 9

Using the Fibrometer instrument,clot detection occurs when:
A)Fibrin strands complete an electric circuit.
B)Fibrin formation increases light absorbance.
C)Fibrin strands decrease ball movement in a magnetic field.
D)Fibrin formation decreases turbidity of the reaction mixture.

Accepted Answer

The Fibrometer detects clot formation when fibrin strands complete an electric circuit, indicating the presence of a clot.

Question 10

Which of the following is a reported parameter for the ADVIA 120 but not for the 	LH 750 instrument?
A)RDW-CV
B)Retic%
C)CHCM
D)MCVr

Accepted Answer

CHCM (Cell Hemoglobin Concentration Mean) is a parameter reported by the ADVIA 120 hematology analyzer but not by the LH 750.

Question 11

Which parameter does the Coulter LH 750 instrument derive from a histogram?
A)Platelet count
B)MCH
C)Leukocyte count
D)Hct

Accepted Answer

The Coulter LH 750 instrument derives platelet count from a histogram.

Question 12

Hydrodynamic focusing is used in automated cell-counting instruments to:
A)Prevent coincidence passage through the detection aperture
B)Increase the visibility of the cells passing through the aperture
C)Allow for maximal light absorbance through the cells passing through the aperture
D)Ensure adequate uptake of fluorescent staining by the cells

Accepted Answer

Hydrodynamic focusing works by using sheath fluid to surround the sample fluid, which prevents cells from passing through the detection aperture at the same time, reducing the likelihood of coincidence events. This results in more accurate cell counting.

Question 13

Which of the following analyzers uses radio frequency to detect and differentiate leukocytes?
A)Bayer Advia 2120
B)Abbott Sapphire
C)Sysmex XE-2100
D)Coulter Gen-S

Accepted Answer

The Sysmex XE-2100 analyzer uses radio frequency to detect and differentiate leukocytes. The other options use different technologies for cell analysis.

Question 14

Choose the correct statement regarding chromogenic analysis in hemostasis testing from the following choices.
A)Chromogenic analysis depends on the selected analyte's ability to cleave a chromophore.
B)Chromogenic analysis is not subject to preanalytical problems because of its mechanism of clot detection.
C)There is no difference between chromogenic analysis and electromechanical detection.
D)Chromogenic analysis depends on the optical detection of ball movement through a steel tube as a clot forms.

Accepted Answer

Chromogenic analysis relies on the analyte's ability to cleave a chromophore, which then results in a color change that can be detected optically. This method is subject to preanalytical problems such as interference from hemolysis or lipemia. Choice B is incorrect because preanalytical problems can still occur with chromogenic analysis. Choice C is also incorrect because electromechanical detection and chromogenic analysis are different methods. Choice D is incorrect because this description is not accurate for chromogenic analysis.

Question 15

Which instrument does not use fluorescence intensity in its determination of reticulocytes?
A)LH 750
B)XE-2100
C)ADVIA 120
D)CELL-DYN Sapphire

Accepted Answer

The LH 750 uses impedance technology to measure reticulocytes, rather than fluorescence intensity.

Question 16

Which of the following instruments is capable of performing electromechanical,optical density,chromogenic,and immunologic methodologies?
A)STArt 4
B)Destiny Plus
C)STA Compact
D)Coag-A-Mate XM

Accepted Answer

The answer of Which of the following instruments is capable...

Question 17

In the reticulocyte determination,using the Sysmex XE-2100,the fluorescent dye containing oxazine and polymethine penetrates the cell membranes and stains the blood cells.What does the oxazine bind to within the cells?
A)Residual DNA
B)Hemoglobin
C)Residual RNA
D)Mitochondria

Accepted Answer

Oxazine binds to residual RNA within the cells in the reticulocyte determination.

Question 18

Using the chromogenic methodology,a coagulation protein's activity level is related to:
A)An increase in turbidity
B)The observed color change
C)The time needed to convert fibrinogen to fibrin
D)A decreased viscosity as plasmin digests fibrin

Accepted Answer

The chromogenic methodology involves the use of a chromogen or substrate that produces a color change when it is cleaved by the target coagulation protein. Therefore, the observed color change is directly related to the activity level of the protein being measured. Choices A, C, and D are not consistent with the chromogenic methodology.

Question 19

Which of the following parameters are calculated on the Coulter Gen-S?
A)Hemoglobin,MCH,MCHC
B)Hematocrit,MCH,MCHC
C)Absolute neutrophil count,RBC count,platelet count
D)RBC count,hematocrit,MCH

Accepted Answer

The Coulter Gen-S measures hematocrit, MCH (mean corpuscular hemoglobin), and MCHC (mean corpuscular hemoglobin concentration) parameters. Hemoglobin and absolute neutrophil count are not measured on this instrument, and while the RBC count is measured, it is not listed as an option in the answer choices.

Question 20

Which parameter does the Sysmex XE-2100 instrument determine by using light-scattering and fluorescence intensity characteristics?
A)Platelet count
B)Erythrocyte count
C)Absolute basophil count
D)Absolute lymphocyte count

Accepted Answer

The answer of Which parameter does the Sysmex XE-2100 instrument...

Quiz 39: Automation in Hematology

Which of the following instruments does not rely on impedance to enumerate cells:

What cellular characteristic is evaluated by radio frequency?

An analyzer is measuring a clot forming by the interaction of a stationary probe with a moving probe in a continuous motion.Suddenly the moving probe stops,and a clot is seen.Name the analyzer.

Regarding the CELL-DYN Sapphire,which parameter is determined by the impedance principle?

Which of the following binds to residual RNA in reticulocytes on the Sysmex XE-2100 analyzer?

Which of the following instruments uses both electromechanical and optical density as a means of clot detection?

Which of the following represents the degree of light scatter that reflects cell complexity on the CELL-DYN Sapphire?

Which of the following analyzers measures reticulocytes by sphering the blood cells prior to analysis?

Using the Fibrometer instrument,clot detection occurs when:

Which of the following is a reported parameter for the ADVIA 120 but not for the LH 750 instrument?

Which parameter does the Coulter LH 750 instrument derive from a histogram?

Hydrodynamic focusing is used in automated cell-counting instruments to:

Which of the following analyzers uses radio frequency to detect and differentiate leukocytes?

Choose the correct statement regarding chromogenic analysis in hemostasis testing from the following choices.

Which instrument does not use fluorescence intensity in its determination of reticulocytes?

Which of the following instruments is capable of performing electromechanical,optical density,chromogenic,and immunologic methodologies?

In the reticulocyte determination,using the Sysmex XE-2100,the fluorescent dye containing oxazine and polymethine penetrates the cell membranes and stains the blood cells.What does the oxazine bind to within the cells?

Using the chromogenic methodology,a coagulation protein's activity level is related to:

Which of the following parameters are calculated on the Coulter Gen-S?

Which parameter does the Sysmex XE-2100 instrument determine by using light-scattering and fluorescence intensity characteristics?