For the reporter protein to be synthesized, the reporter gene must be fused in the proper codon reading frame with respect to:

Question

Quizplus · Accepted Answer

The promoter is the DNA sequence that initiates transcription, so the reporter gene must be fused in the proper reading frame with respect to the promoter to ensure the correct transcription and translation of the reporter protein. The ribosome binding site is important for translation initiation, but the reading frame with respect to this site is only important for the coding sequence of the target gene, not the reporter gene. The vector origin of replication and the target gene are also not directly related to the reading frame of the reporter gene.

For the Reporter Protein to Be Synthesized, the Reporter Gene