Why Must Double-Stranded DNA Probes Be Denatured When Used in Northern

Why must double-stranded DNA probes be denatured when used in Northern blot experiments?

A) To ensure that they are properly radioactively labeled or tagged with a dye.
B) To ensure that the DNA coding strand hybridizes to RNA on the membrane.
C) To ensure that both DNA strands bind the single-stranded RNA on the membrane.
D) All of the above.
E) None of the above.