A major advantage of real-time PCR is that it can be used to quantify the original amount of RNA in a sample. What additional step must be accomplished in this particular application?

Question

Quizplus · Accepted Answer

Real-time PCR requires the use of complementary DNA (cDNA) as a template for amplification. Therefore, RNA must first be converted to cDNA via reverse transcription before PCR can be performed. This step is necessary to ensure accurate quantification of RNA in the sample.

A Major Advantage of Real-Time PCR Is That It Can