In the yeast two-hybrid analysis technique, in vivo protein-protein interactions are explored. The "bait" and "prey" proteins are translationally fused to the cleaved activation and DNA-binding domains of the GAL4 transcription factor. What is the target gene of GAL4?

Question

Quizplus · Accepted Answer

The target gene of GAL4 in yeast is gal1, which is involved in galactose metabolism. GAL4 is a transcription factor that binds to the upstream activating sequence of the GAL1 gene to activate its transcription.

In the Yeast Two-Hybrid Analysis Technique, in Vivo Protein-Protein Interactions