The fluorescent proteins YFP and CFP emit yellow and blue (cyan) light, respectively. These and other variations of GFP have been obtained through:

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The fluorescent proteins YFP and CFP are obtained through mutation of the gfp gene in specific sites. This process involves altering the DNA sequence of the gfp gene, resulting in changes to the structure and function of the GFP protein. By carefully selecting which sites to mutate, scientists can create GFP variants that emit different colors of light, such as yellow, blue, and even red. This technique is known as site-directed mutagenesis and is a common method for modifying the properties of proteins in biotechnology and molecular biology.

The Fluorescent Proteins YFP and CFP Emit Yellow and Blue