Question 1

The 3¢ ends of RNA transcripts synthesized by RNA polymerase II are produced by: 1.Endonucleolytic cleavage of the primary transcripts
2)Termination of transcription
3)The addition of the 5' 7-methyl guanosine cap

Accepted Answer

The 3' ends of RNA transcripts synthesized by RNA polymerase II are produced by both endonucleolytic cleavage of the primary transcripts and termination of transcription.

Question 2

Briefly describe the process of initiation for RNA transcription in prokaryotes

Accepted Answer

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Question 3

Introns in mRNA are removed by:

Accepted Answer

Introns in mRNA are removed by ribonucleoprotein structures called spliceosomes. These spliceosomes consist of small nuclear RNAs (snRNAs) and proteins that form complexes and recognize specific site sequences at intron-exon boundaries. They carry out the actual cutting and splicing of the pre-mRNA to remove the introns and join together the exons.

Question 4

Noncoding sequences found in eukaryotic genes that interrupt the coding sequences are known as:

Accepted Answer

Noncoding sequences found in eukaryotic genes that interrupt the coding sequences are known as introns. Exons are the coding sequences that are spliced together after the introns are removed. Spliceosomes are large molecular complexes responsible for removing introns and splicing exons together. Pre-mRNA is the initial transcript of a gene before it is processed to become mature mRNA.

Question 5

Introns in tRNA are removed by:

Accepted Answer

Introns in tRNA are specifically removed by the concerted action of a splicing endonuclease and ligase. The endonuclease cuts out the intron, and the ligase joins the two exons together.

Question 6

Compare,in detail,the three forms of introns removal in eukaryotes.

Accepted Answer

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Question 7

How does transcription and RNA processing in eukaryotes differ from the same processes in prokaryotes?

Accepted Answer

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Question 8

Compare and contrast rho-independent termination and rho-dependent termination during transcription in prokaryotes

Accepted Answer

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Question 9

The snRNA responsible for the recognition/binding of the 5' splice site prior to the initial cleavage reaction is:

Accepted Answer

The U1 snRNA is responsible for recognizing and binding to the 5' splice site during mRNA splicing. It base pairs with the intron sequence adjacent to the splice site and helps to define the boundaries of the intron.

Question 10

The promoter region in E.coli is comprised of two traditionally recognized sequences.Describe these sequences,their properties,and their purposes.

Accepted Answer

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Question 11

Which of the following is a form of RNA editing? 1.The polyadenylation of the pre-mRNA sequence
2)The changing of the structures of individual bases
3)The insertion or deletion of uridine monophosphate residues

Accepted Answer

RNA editing involves the alteration of nucleotide sequences in RNA, which includes changing the structures of individual bases and the insertion or deletion of uridine monophosphate residues. Polyadenylation, although a modification of RNA, is not considered a form of RNA editing.

Question 12

How does initiation of transcription in eukaryotes differ from initiation of transcription in prokaryotes?

Accepted Answer

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Quiz 11: Transcription And RNA Processing

The 3¢ ends of RNA transcripts synthesized by RNA polymerase II are produced by: 1.Endonucleolytic cleavage of the primary transcripts 2) Termination of transcription 3) The addition of the 5' 7-methyl guanosine cap