When choosing a restriction enzyme for use in recombinant DNA technologies,it is often preferred that the enzyme generate cohesive,or "sticky," ends.Why is this preferred?
A)The sticky ends prevent the DNA from re- annealing to any DNA.
B)The sticky ends make the DNA bind tighter to any cut DNA.
C)The sticky ends stick to the purification medium making the fragments easier to purify.
D)The sticky ends have hydrogen bonds that help re- anneal the cut DNA.
E)The sticky ends do not have hydrogen bonds to help in re- annealing the cut DNA.

Question

Quizplus · Accepted Answer

Sticky ends have overhanging sequences that can form hydrogen bonds with complementary sequences on another DNA molecule, facilitating the re-annealing or joining of DNA fragments during the cloning process.

When Choosing a Restriction Enzyme for Use in Recombinant DNA