If one wishes to clone a gene using typical restriction endonucleases,how does the restriction endonuclease identify the appropriate cut sites in the genome?
A)The endonuclease cannot identify the cut sites.
B)The endonuclease recognizes the gene of interest.
C)The endonuclease cuts randomly in the genome.
D)The endonuclease identifies its specific recognition sequence.

Question

Quizplus · Accepted Answer

Restriction endonucleases identify their specific recognition sequence, usually a palindromic sequence of bases, in the genome and cut the DNA at that site. This specific recognition sequence is what allows researchers to target specific genes for cloning.

If One Wishes to Clone a Gene Using Typical Restriction