The following are four processes common to most cloning experiments: i)transforming bacteria
Ii)plating bacteria on selective medium
Iii)cutting DNA with restriction endonucleases
Iv)ligating DNA fragments
Place the components of this list in the order in which they would most likely occur during a cloning experiment
A)iii,iv,i,ii
B)i,ii,iii,iv
C)ii,iii,i,iv
D)iv,i,iii,ii
E)ii,i,iv,iii

Question

Quizplus · Accepted Answer

The correct sequence for a typical cloning experiment is: iii) cutting DNA with restriction endonucleases to create fragments, iv) ligating DNA fragments to insert them into vectors, i) transforming bacteria with the recombinant DNA, and ii) plating bacteria on selective medium to identify those that have taken up the recombinant DNA.

The Following Are Four Processes Common to Most Cloning Experiments