When a 5- kb circular plasmid is digested with a restriction enzyme that has three recognition sites on the plasmid,how many bands can be visualized on an agarose gel?
A)1
B)2
C)3
D)4

Question

Quizplus · Accepted Answer

If there are three recognition sites on the plasmid, the enzyme would cut the plasmid into four fragments. Therefore, there would be three bands visible on the gel representing the three fragments of different sizes.

When a 5- Kb Circular Plasmid Is Digested with a Restriction