Next- generation sequencing takes advantage of the concept of sequencing- by- synthesis.What is sequence- by- synthesis?
A)The use of PCR to incorporate ddNTPs,followed by electrophoresis.
B)The use of a multiwell system and microfluidics to detect incorporated nucleotides at each base.
C)The use of firefly luciferase and pyrophosphate to report on nucleotide incorporation.
D)The use of a single polymerase in a nanopore that reports on a single molecule of synthesized DNA.

Question

Quizplus · Accepted Answer

Sequence-by-synthesis involves detecting the incorporation of nucleotides during DNA synthesis, often using a multiwell system and microfluidics to monitor each base addition.

Next- Generation Sequencing Takes Advantage of the Concept of Sequencing-