Sanger sequencing is based on the order in which ddNTPs are added to a growing polynucleotide.Why are ddNTPs integral to the Sanger sequencing method?
A)They have a 3' hydroxyl that allows for extension of the polynucleotide.
B)They do not have a 2' hydroxyl,which does not allow the extension of the polynucleotide.
C)They do not have a 3' hydroxyl,which does not allow the extension of the polynucleotide.
D)They have a 2' hydroxyl that allows for extension of the polynucleotide.

Question

Quizplus · Accepted Answer

ddNTPs (dideoxynucleotides) are integral to the Sanger sequencing method because they lack a 3' hydroxyl group. This absence prevents further extension of the polynucleotide chain once a ddNTP is incorporated, allowing for the determination of the sequence by terminating synthesis at specific nucleotides.

Sanger Sequencing Is Based on the Order in Which DdNTPs