When performing a sequencing reaction using Sanger sequencing,which ddNTPs must be included in the reaction?
A)ddGTP
B)ddCTP
C)ddATP
D)ddTTP
E)all four ddNTPs must be present

Question

Quizplus · Accepted Answer

All four ddNTPs must be included in the reaction as they terminate the chain at different positions and allow for the determination of the nucleotide sequence.

When Performing a Sequencing Reaction Using Sanger Sequencing,which DdNTPs Must