What is the function of a ddNTP in DNA sequencing?
A)termination of DNA synthesis
B)methylation of guanine
C)provide a 3' hydroxyl for continued elongation
D)enhancing the processivity of the polymerase

Question

Quizplus · Accepted Answer

ddNTPs, or dideoxynucleotide triphosphates, lack a 3' hydroxyl group which is required for the formation of a phosphodiester bond with the next incoming nucleotide during DNA synthesis. Therefore, when a ddNTP is incorporated by a DNA polymerase during sequencing, it terminates (ends) the extension of the DNA strand. This results in the synthesis of a population of DNA fragments that differ in length by one nucleotide, allowing for the determination of the DNA sequence.

What Is the Function of a DdNTP in DNA Sequencing