The percentage of CD4 positive lymphocytes is determined by flow cytometry using staining with fluorescent-tagged antibodies against CD3 and CD4 followed by whole blood RBC lysis. What calculations does the dual platform use to determine the absolute number of CD4 positive cells?
A) Absolute CD4 count 100
B) Number of CD4+ cells 100
C) Absolute lymphocyte count % CD4+ lymphocytes/100
D) % lymphocytes % CD4+ lymphocytes/100

Question

Quizplus · Accepted Answer

The dual platform uses the calculation of absolute lymphocyte count % CD4+ lymphocytes/100 to determine the absolute number of CD4 positive cells. This takes into account the percentage of CD4 positive lymphocytes in relation to the total lymphocyte count.

The Percentage of CD4 Positive Lymphocytes Is Determined by Flow