Prior to using PCR to introduce mutations into DNA in vitro,what was a common method of mutagenesis used by scientists?
A)Self-fertilization of plants or inbreeding of animal models
B)Chemicals to induce mutations
C)Physical manipulation of DNA (e.g.vortexing)
D)Using only old parents to set crosses in plants and animals

Question

Quizplus · Accepted Answer

Chemicals were often used by scientists as mutagens prior to the invention of PCR. Common mutagens included ethyl methanesulfonate (EMS) and nitrous acid. These agents were used to induce random mutations in DNA that could then be studied to better understand gene function.

Prior to Using PCR to Introduce Mutations into DNA in Vitro,what