Select the experiments that can be characterized as reverse genetics: (check all that apply)
A)Generation and analysis of a knockout mouse
B)Random mutagenesis of an organism,then selecting mutants by phenotype
C)RNA interference of a gene in C.elegans
D)Using reverse transcriptase to create a cDNA library

Question

Quizplus · Accepted Answer

Reverse genetics involves using genetic tools to elucidate the function of a gene or protein. Generating a knockout mouse involves deleting or disrupting a gene to observe the resulting phenotype, which is a classic example of reverse genetics. RNA interference (RNAi) is another form of reverse genetics where the expression of a gene is reduced or eliminated using small RNA molecules. Random mutagenesis (B) and creating a cDNA library (D) are not considered reverse genetics because they do not involve manipulating a specific gene or protein to understand its function.

Select the Experiments That Can Be Characterized as Reverse Genetics