Which component of the PCR mix do researchers manipulate to induce a site-specific mutation?
A)Primers
B)Taq polymerase
C)DNA template
D)Reverse transcriptase

Question

Quizplus · Accepted Answer

Researchers can manipulate the primers in the PCR mix to introduce site-specific mutations. By changing the sequence of the primers, they can create a mutation at a specific location within the DNA template. Taq polymerase and reverse transcriptase are enzymes used in PCR or reverse transcription PCR, respectively, but they are not manipulated to induce site-specific mutations. The DNA template is the starting material that is amplified in PCR, and it is not manipulated to introduce mutations.

Which Component of the PCR Mix Do Researchers Manipulate to Induce