The PCR technique requires a DNA polymerase from an organism that can endure high heat,such as Thermus aquaticus.What step of the protocol makes the heat-stability most necessary?
A)Synthesis
B)Primer annealing
C)Denaturation
D)Antibody binding

Question

Quizplus · Accepted Answer

Denaturation is the step in which the double-stranded DNA template is heated to a high temperature to separate the two strands. This requires a DNA polymerase that can withstand the high temperature, such as Taq polymerase from Thermus aquaticus.

The PCR Technique Requires a DNA Polymerase from an Organism