When analyzing the production of dTMP by thymidylate synthase in a cell-free system, which of the following compounds would be found as an additional product of this enzyme? A) 7,8-dihydrofolate B) NADP⁺ C) N⁵,N¹⁰-methylenetetrahydrofolate D) glycine

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Quizplus · Accepted Answer

Thymidylate synthase uses 5,10-methylenetetrahydrofolate as a substrate to make dTMP, and in the process, it converts 5,10-methylenetetrahydrofolate to dihydrofolate. Dihydrofolate is then reduced to 7,8-dihydrofolate by dihydrofolate reductase. Therefore, in a cell-free system producing dTMP by thymidylate synthase, 7,8-dihydrofolate would be found as an additional product. NADPH is not involved in the reaction, and the other compound listed is a complex mixture and unclear what it pertains to. Glycine is not involved in the biosynthesis of dTMP.

When Analyzing the Production of DTMP by Thymidylate Synthase in a Cell-Free