Which technique ionizes polypeptides by embedding the tryptic fragments into a light-absorbing matrix and exposing it to a laser?
A) ESI
B) MALDI
C) NMR
D) X-ray crystallography

Question

Quizplus · Accepted Answer

The technique described is MALDI (Matrix-Assisted Laser Desorption/Ionization). It is commonly used in mass spectrometry to ionize and analyze large biomolecules such as polypeptides, proteins and nucleic acids. The tryptic fragments are embedded into a light-absorbing matrix, which enhances the formation of ions upon exposure to laser irradiation.

Which Technique Ionizes Polypeptides by Embedding the Tryptic Fragments into a Light-Absorbing