Using the CRISPR/Cas9 system, a genetically-engineered sgRNA complementary to a target site in the genome binds to Cas9 endonuclease.A mutation can occur at this site when
A)Cas9 cleaves the DNA and nonhomologous end-joining results in a small deletion.
B)Cas9 cleaves the DNA and homologous recombination with the homologous chromosome is used to repair the break.
C)Cas9 cleaves the DNA to remove the complementary sequence.
D)Cas9 cleaves the DNA and nonhomologous end-joining correctly repairs the break.

Question

Quizplus · Accepted Answer

When Cas9 cleaves the DNA, nonhomologous end-joining (NHEJ) can result in small insertions or deletions (indels) at the site, leading to mutations.

Using the CRISPR/Cas9 System, a Genetically-Engineered SgRNA Complementary to a Target