Researchers combined two yeast strains of opposite mating type; the a strain was resistant to chloramphenicol and the ? strain was sensitive to chloramphenicol. The diploid progeny were allowed to undergo several cell divisions, and then the cells were replica plated on glycerol medium with and without chloramphenicol.
-What results would you expect if mitochondria are inherited uniparentally from the a strain?
A)Some, but not all, yeast colonies found on the plate without chloramphenicol will also grow on the plate with chloramphenicol.
B)All of the yeast colonies on the plate without chloramphenicol will also grow on the plate with chloramphenicol.
C)Many yeast colonies will grow on the plate without chloramphenicol and no colonies will grow on the plate with chloramphenicol.
D)No colonies will grow on either plate.

Question

Quizplus · Accepted Answer

If mitochondria are inherited uniparentally from the a strain, which is resistant to chloramphenicol, all diploid progeny would inherit this resistance. Therefore, all yeast colonies able to grow on the plate without chloramphenicol (indicating they are alive and viable) would also be able to grow on the plate with chloramphenicol, as they all inherit the chloramphenicol resistance from the a strain mitochondria.

Researchers Combined Two Yeast Strains of Opposite Mating Type; the a Strain