Which is part of a DNA sequencing technique that is useful for high-throughput sequencing, but is not part of Sanger DNA sequencing?
A)Using dideoxynucleotides as chain terminators
B)Separating DNA by size using gel electrophoresis
C)Hybridization between the template and a primer
D)Removal of a chemical group that blocks the 3′ end of the new DNA strand
E)Labeling deoxynucleotides with fluorescent tags

Question

Quizplus · Accepted Answer

Sanger sequencing does not involve the removal of a chemical group that blocks the 3′ end of the new DNA strand. This step is characteristic of some high-throughput sequencing techniques, such as Illumina sequencing, where reversible terminator bases are used.

Which Is Part of a DNA Sequencing Technique That Is