How can microarrays differentiate between a wild-type allele and a disease allele that differ at only one nucleotide?
A)The DNA sequences of both alleles are determined.
B)Oligonucleotides hybridize with the two alleles differently.
C)Polymerase chain reaction primers amplify DNA from one allele, but not the other.
D)Microarrays are not capable of detecting a difference between those alleles.

Question

Quizplus · Accepted Answer

Oligonucleotides on a microarray can be designed to specifically hybridize to sequences with even a single nucleotide difference, allowing differentiation between a wild-type allele and a disease allele that differ by only one nucleotide.

How Can Microarrays Differentiate Between a Wild-Type Allele and a Disease