Ninhydrin is primarily used to detect amino acids, as it reacts with free amino groups to produce a deep blue or purple color, which is especially useful for visualizing amino acids derived from the hydrolysis of peptides.

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Ninhydrin Is Used

Question 36

Multiple Choice

Ninhydrin is used

A) to determine the N-terminal amino acid of a peptide.
B) to sequence amino acids in a peptide.
C) to detect amino acids derived from hydrolysis of a peptide.
D) to couple amino acids during solid-phase peptide synthesis.
E) to hydrolyze peptides on the carboxyl side of aromatic amino acid residues.

Correct Answer: