Question 1

Restriction enzymes are useful only on synthetic DNAs.

Accepted Answer

Restriction enzymes are naturally occurring enzymes that recognize and cut specific sequences of DNA, regardless of whether they are synthetic or naturally occurring. They are widely used in molecular biology for tasks such as cloning, gene manipulation, and DNA profiling.

Question 2

Explain what a transgenic organism is, and give two examples.

Accepted Answer

The answer of Explain what a transgenic organism is, and...

Question 3

Discuss one of the pros and cons regarding the application of recombinant DNA technology.

Accepted Answer

The answer of Discuss one of the pros and cons...

Question 4

Gene therapy for human genetic diseases has not been successful yet.

Accepted Answer

While there have been some successful cases of gene therapy for certain genetic diseases, there is still much research and development required to make it widely effective and safe for all patients.

Question 5

Outline a procedure for producing a useful new recombinant product.

Accepted Answer

The answer of Outline a procedure for producing a useful...

Question 6

Under ideal conditions, the number of DNA molecules produced during PCR increases exponentially.

Accepted Answer

This is because PCR involves repeated cycles of denaturation, annealing, and extension which leads to a doubling of the number of DNA molecules each cycle. This results in an exponential increase in the number of DNA molecules produced over time.

Question 7

Reverse transcriptase synthesizes a DNA molecule from an RNA template.

Accepted Answer

Reverse transcriptase is an enzyme that can synthesize a DNA molecule from an RNA template, a process called reverse transcription. This is a key step in the replication of retroviruses, such as HIV, which use RNA as their genetic material. The resulting DNA molecule is known as complementary DNA (cDNA).

Question 8

Sticky- end fragments generated by EcoRI will hydrogen bond to any other sticky- end sequence.

Accepted Answer

Sticky-end fragments generated by EcoRI are specific in their sequence and will only hydrogen bond to complementary sticky-end sequences, not just any sequence.

Question 9

Discuss some of the impacts that tools and techniques of recombinant DNA technology have had on medicine.

Accepted Answer

The answer of Discuss some of the impacts that tools...

Question 10

A subunit vaccine is prepared by extensive manipulation of the genome of the pathogen.

Accepted Answer

A subunit vaccine is made using only specific pieces of the pathogen, such as proteins or sugars, not by manipulating its genome.

Question 11

A collection of bacterial clones each of which contains a portion of the gene sequences of an organism is known as a microarray of that organism's genome.

Accepted Answer

A collection of bacterial clones containing portions of an organism's gene sequences is known as a genomic library, not a microarray. A microarray is a tool used to analyze gene expression.

Question 12

DNA fingerprinting produces a unique banding pattern of DNA fragments for comparison with other DNA samples.

Accepted Answer

DNA fingerprinting analyzes specific regions of DNA that vary greatly among individuals, resulting in a unique pattern of bands when visualized, allowing for comparison between different DNA samples.

Question 13

Compare and contrast the two major categories of restriction enzymes.

Accepted Answer

The answer of Compare and contrast the two major categories...

Question 14

The goals of recombinant DNA technology include production of new organisms with useful combinations of traits.

Accepted Answer

Recombinant DNA technology allows for the manipulation and combination of genetic material from different organisms, leading to the production of new organisms with desired traits.

Question 15

Southern blotting is a technique that can be used to identify microbes that cannot be cultured.

Accepted Answer

The answer of Southern blotting is a technique that can...

Quiz 8: Recombinant Dna Technology