If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure, what would be the result?
A) Many mistakes would occur.
B) DNA replication would occur twice as fast as normal.
C) DNA replication would occur more slowly than normal.
D) DNA replication would not occur at all.
E) DNA replication would stop after one cycle.

Question

Quizplus · Accepted Answer

Escherichia coli DNA polymerase is not thermostable and would denature at the high temperatures used in PCR, stopping DNA replication after one cycle.

If a Researcher Used Escherichia Coli DNA Polymerase Instead of Thermus