Which of the following would be an appropriate sequence of temperatures for PCR?
A) 65°C, 72°C, 94°C
B) 94°C, 37°C, 55°C
C) 94°C, 65°C, 72°C
D) 94°C, 55°C, 37°C
E) 72°C, 65°C, 94°C

Question

Which of the following would be an appropriate sequence of temperatures for PCR? 
A) 65°C, 72°C, 94°C
B) 94°C, 37°C, 55°C
C) 94°C, 65°C, 72°C
D) 94°C, 55°C, 37°C
E) 72°C, 65°C, 94°C

Quizplus · Accepted Answer

PCR involves three main steps: denaturation at around 94°C, annealing at a lower temperature (commonly around 55°C-65°C), and extension at 72°C.

Which of the Following Would Be an Appropriate Sequence of Temperatures