Which of the following procedures might be used to detect the presence of genetic sequences of a virus in a sample?
A) Southern blotting
B) genome mapping
C) PCR
D) creation of a gene library
E) Southern blotting or PCR

Question

Quizplus · Accepted Answer

Southern blotting and PCR are both techniques that can be used to detect the presence of genetic sequences of a virus in a sample. Southern blotting involves the separation of DNA fragments by gel electrophoresis and then transferring them to a nitrocellulose membrane. The membrane is then probed with a labeled DNA sequence that is complementary to the target sequence. If the target sequence is present in the sample, it will hybridize to the probe and can be detected. PCR involves the amplification of a specific DNA sequence using a pair of primers that are complementary to the target sequence. The PCR reaction is carried out in a thermal cycler, which cycles the reaction mixture through a series of temperature changes that allow the primers to anneal to the target sequence, extend, and then denature. The PCR products can then be analyzed by gel electrophoresis to determine if the target sequence is present in the sample.

Which of the Following Procedures Might Be Used to Detect