Question 1

Is the accompanying figure DNA or RNA? Is the circle closer to the 5' or 3' end?

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Question 2

List three forms of DNA.

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Question 3

Explain how and why the following circumstances influence characteristics of temperature -induced DNA melting:
percentage of GC base pairs
urea (forms hydrogen bonds with bases)
sodium chloride (neutralizes negatively charged phosphates)

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Question 4

If the GC content of a DNA molecule is 60 percent, what are the molar percentages of the four bases (G, C, T,
A)?

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Question 5

What is meant by the term antiparallel?

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Question 6

At what approximate wavelengths do DNA, RNA, and proteins maximally absorb light?

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Question 7

The transforming principle discovered by Griffith is RNA.

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The transforming principle discovered by Griffith is DNA, not RNA.

Question 8

Consider the structure of double -stranded DNA. When DNA is placed into distilled water, it denatures; however, by adding NaCl, the DNA renatures. Why?

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Question 9

During the polymerization of nucleic acids, covalent bonds are formed between neighboring nucleotides. Which carbons are involved in such bonds?

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Question 10

Is the accompanying figure DNA or RNA? Is the circle closer to the 5' or 3' end?

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Question 11

Assume that the molar percentage of thymine in a double -stranded DNA is 20. What are the percentages of the four bases (G, C, T,
A)? If the DNA is single -stranded, would you change your answer?

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Question 12

What is the difference between a polynucleotide and an oligonucleotide?

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Question 13

What does it mean to say that double -stranded nucleic acids are antiparallel?

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Question 14

All other factors being equal, the renaturation of the three classes of complementary nucleic acid sequences occurs in what order, from fastest to slowest?

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Question 15

Suppose that the dinucleotide in the accompanying figure were cleaved with the
enzyme spleen diesterase, which breaks the covalent bond connecting the phosphate to C -5'. After such cleavage, to which nucleoside is the phosphate now attached?

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Question 16

Name the pyrimidines and the purines in DNA.

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Question 17

(a) Assume that A + T/G + C equals 0.5 in one strand of DNA. What is the ratio of these bases in the complementary strand? (b) If A + G/T + C equals 0.5 in one strand, what is the ratio of these bases in the complementary strand?

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Question 18

The base content of a sample of DNA is as follows: A = 31%, G = 31%, T = 19%, C =
19%. What conclusion can be drawn from this information?

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Question 19

Provide an overview of the structure of Z -DNA.

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Question 20

What is the name of the precursor molecule used in nucleic acid synthesis?

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Quiz 9: DNA Structure and Analysis

Is the accompanying figure DNA or RNA? Is the circle closer to the 5' or 3' end?

List three forms of DNA.

Explain how and why the following circumstances influence characteristics of temperature -induced DNA melting: percentage of GC base pairs urea (forms hydrogen bonds with bases) sodium chloride (neutralizes negatively charged phosphates)

If the GC content of a DNA molecule is 60 percent, what are the molar percentages of the four bases (G, C, T, A)?

What is meant by the term antiparallel?

At what approximate wavelengths do DNA, RNA, and proteins maximally absorb light?

The transforming principle discovered by Griffith is RNA.

Consider the structure of double -stranded DNA. When DNA is placed into distilled water, it denatures; however, by adding NaCl, the DNA renatures. Why?

During the polymerization of nucleic acids, covalent bonds are formed between neighboring nucleotides. Which carbons are involved in such bonds?

Is the accompanying figure DNA or RNA? Is the circle closer to the 5' or 3' end?

Assume that the molar percentage of thymine in a double -stranded DNA is 20. What are the percentages of the four bases (G, C, T, A)? If the DNA is single -stranded, would you change your answer?

What is the difference between a polynucleotide and an oligonucleotide?

What does it mean to say that double -stranded nucleic acids are antiparallel?

All other factors being equal, the renaturation of the three classes of complementary nucleic acid sequences occurs in what order, from fastest to slowest?

Suppose that the dinucleotide in the accompanying figure were cleaved with the enzyme spleen diesterase, which breaks the covalent bond connecting the phosphate to C -5'. After such cleavage, to which nucleoside is the phosphate now attached?

Name the pyrimidines and the purines in DNA.

(a) Assume that A + T/G + C equals 0.5 in one strand of DNA. What is the ratio of these bases in the complementary strand? (b) If A + G/T + C equals 0.5 in one strand, what is the ratio of these bases in the complementary strand?

The base content of a sample of DNA is as follows: A = 31%, G = 31%, T = 19%, C = 19%. What conclusion can be drawn from this information?

Provide an overview of the structure of Z -DNA.

What is the name of the precursor molecule used in nucleic acid synthesis?