Question 1

In a typical PCR, primers are used to cleave specific regions of the DNA template.

Accepted Answer

Primers are used to initiate DNA synthesis at specific regions of the DNA template, not to cleave it.

Question 2

In recombinant DNA technology, YAC, RFLP, and h have identical uses.

Accepted Answer

YAC (Yeast Artificial Chromosome) is used for cloning large DNA fragments, RFLP (Restriction Fragment Length Polymorphism) is a technique for analyzing DNA variations, and "h" is not a recognized term in this context. They serve different purposes in genetic research.

Question 3

The main purpose of a probe is its insertion in plasmid DNA.

Accepted Answer

The main purpose of a probe is to detect specific DNA sequences, not to insert into plasmid DNA.

Question 4

Reverse transcriptase is often used as the heat -stable enzyme in PCR.

Accepted Answer

PCR typically uses a heat-stable DNA polymerase, such as Taq polymerase, rather than reverse transcriptase which is an enzyme used to generate cDNA from RNA.

Question 5

In a PCR, primers are complementary to stretches of DNA with which they anneal.

Accepted Answer

Primers in a PCR are designed to be complementary to specific stretches of DNA that flank the target sequence to be amplified. This allows them to anneal to the appropriate location and serve as a starting point for DNA polymerase to extend and amplify the sequence.

Question 6

During a PCR, heat is provided to inactivate the polymerase enzyme.

Accepted Answer

Heat is not provided to inactivate the polymerase enzyme during a PCR. The enzyme is instead denatured by increasing the temperature to around 95°C before each new cycle of amplification.

Question 7

The function of a ddNTP in DNA sequencing is to methylate guanine.

Accepted Answer

The function of a ddNTP (dideoxynucleotide triphosphate) in DNA sequencing is to terminate the extension of the DNA strand by preventing the addition of another nucleotide to the 3' end. This results in a mixture of fragments of different lengths that can be separated and analyzed to determine the sequence of the original DNA template. ddNTPs do not methylate guanine.

Question 8

E. coli is a common YAC.

Accepted Answer

E. coli is a type of bacteria, not a YAC (yeast artificial chromosome).

Question 9

In recombinant DNA technology, a YAC is an enzyme isolated from a large South American, four -legged mammal.

Accepted Answer

YAC stands for yeast artificial chromosome, which is a synthetic chromosome created by inserting and manipulating DNA in yeast cells. It has nothing to do with a South American mammal.

Question 10

A restriction map provides the location of sites cleaved by restriction enzymes.

Accepted Answer

A restriction map is a diagram that shows the location of sites cleaved by restriction enzymes on a DNA molecule. These sites can be visualized by running the DNA fragments on a gel and measuring their size. Restriction maps are important tools for molecular biology research, as they provide information about the structure and organization of DNA molecules.

Quiz 17: Recombinant Dna Technology