Which of the following limits the utility of yeast two-hybrid screens in identifying protein interactions?
A) Yeast two-hybrid screens can only be applied to bait proteins that are less than 20kDa.
B) Only two proteins can be tested for their ability to interact,per day.
C) Yeast two-hybrid screens can only find proteins that interact with transcription factors.
D) Fusion proteins must be affinity purified.
E) Bait proteins may not fold properly when fused to other proteins.

Question

Quizplus · Accepted Answer

Yeast two-hybrid screens may not be completely reliable in identifying protein interactions because the bait proteins may not fold correctly when fused to other proteins, preventing the detection of true interactions.

Which of the Following Limits the Utility of Yeast Two-Hybrid