Which of the following is not directly involved in ICAT (isotope coded affinity tag)labelling and purification?
A) cysteine
B) deuterium
C) avidin
D) biotin
E) antibodies

Question

Quizplus · Accepted Answer

ICAT labeling and purification involve specifically targeting cysteine residues (A) in proteins, using biotin (D) as a tag for purification, and sometimes isotopes like deuterium (B) for differential labeling. Avidin (C) is used for its strong affinity to biotin, facilitating the purification process. Antibodies (E) are not directly involved in the ICAT process, which relies on chemical tagging and affinity purification rather than immune recognition.

Which of the Following Is Not Directly Involved in ICAT