In a typical yeast two-hybrid experiment,which of the following occurs when bait and prey interact?
A) HIS3 binds to the promoter of a reporter gene.
B) Gal4 activator and binding domains form a complex.
C) HIS3 and Gal4 form a complex.
D) HIS3 transcription is blocked.
E) Expression of Gal4 is activated.

Question

Quizplus · Accepted Answer

In a yeast two-hybrid experiment, the bait and prey proteins interact to bring together the Gal4 DNA-binding domain and the Gal4 activation domain, forming a complex that activates the transcription of a reporter gene (usually lacZ or HIS3). Choice B correctly describes this process, as it involves the formation of the Gal4 activator and binding domains complex.

In a Typical Yeast Two-Hybrid Experiment,which of the Following Occurs