In a typical yeast two-hybrid experiment,which of the following is true of a cell that contains an interaction between bait and prey?
A) No growth on media that contains His,and no growth on media that contains Gal
B) No growth on media that lacks His,and no growth on media that lacks Gal
C) Growth on media that lacks His,and no growth on media that contains His
D) Growth on media that contains His,and no growth on media that lacks His
E) Growth on media that lacks His,and growth on media that contains His

Question

Quizplus · Accepted Answer

In a yeast two-hybrid experiment, the interaction between bait and prey proteins activates the transcription of a reporter gene, allowing the yeast to grow on media lacking histidine (His). The presence or absence of galactose (Gal) is not directly relevant to the interaction between bait and prey in this context; thus, the yeast's ability to grow is not affected by Gal but by the presence of the interaction that enables growth on media lacking His.

In a Typical Yeast Two-Hybrid Experiment,which of the Following Is