What is an advantage of affinity purification-based methods over yeast two-hybrid (Y2H)methods,when screening for interacting proteins?
A) Affinity methods isolate molecules from their native context and do not require recombinant protein production.
B) Affinity methods use fusion proteins rather than antibodies,which are more expensive to produce.
C) Affinity methods,but not Y2H,can be used to find proteins that interact with transcription factors.
D) Proteins used in affinity methods do not need to be properly folded.
E) Affinity methods can be applied to proteins from a wider range of eukaryotes than can be used in Y2H.

Question

Quizplus · Accepted Answer

Affinity purification-based methods isolate interacting molecules from their native context, allowing for more accurate identification of interacting partners. Additionally, affinity methods do not require recombinant protein production, which can be time-consuming and expensive.

What Is an Advantage of Affinity Purification-Based Methods Over Yeast