What is the role of HIS3 in yeast two-hybrid experiments?
A) It is a reporter gene.
B) It is required for growth in media that contain histidine.
C) It activates transcription.
D) It binds DNA.
E) It acts as a bait for other proteins.

Question

Quizplus · Accepted Answer

HIS3 is used as a reporter gene in yeast two-hybrid experiments to indicate successful protein-protein interactions. When the interaction occurs, HIS3 is activated, allowing yeast to grow in media lacking histidine.

What Is the Role of HIS3 in Yeast Two-Hybrid Experiments