Which of the following would be required for affinity purification but not for yeast two-hybrid (Y2H)?
A) A fusion protein including the protein of interest.
B) A fluorescent reporter gene.
C) A selectable marker.
D) An antibody.
E) An antibiotic.

Question

Quizplus · Accepted Answer

Affinity purification requires an antibody for specifically binding the protein of interest and purifying it from the mixture. In contrast, yeast two-hybrid does not require an antibody but instead relies on the reconstitution of a transcriptional activator to detect protein-protein interactions. The other options listed (A, B, C, and E) are not specific to either affinity purification or yeast two-hybrid and may be required or not required depending on the specific experimental setup.

Which of the Following Would Be Required for Affinity Purification