In the Southern blot technique, which of the following is NOT required?
A) electrophoresis to separate fragments
B) transfer of DNA to nitrocellulose
C) addition of a labeled probe to identify the gene of interest
D) restriction enzyme digestion of DNA
E) addition of heat-stable DNA polymerase to amplify DNA

Question

Quizplus · Accepted Answer

The Southern blot technique does not require the addition of heat-stable DNA polymerase, as it is not a PCR-based method and does not involve DNA amplification.

In the Southern Blot Technique, Which of the Following Is