A direct ELISA is most useful for detection of antigens while an indirect ELISA is used to determine if a patient has a particular type of antibody in their blood.

Question

Quizplus · Accepted Answer

In a direct ELISA, the antigen is immobilized on the plate and directly detected by an antibody linked to a detectable enzyme, making it useful for antigen detection. In contrast, an indirect ELISA involves an antigen attached to the plate and the detection of specific antibodies in a patient's sample using a secondary antibody-enzyme conjugate, thus it's used for determining the presence of specific antibodies.

A Direct ELISA Is Most Useful for Detection of Antigens