In Which Order Should the Following Steps Be Done to Identify

In which order should the following steps be done to identify a quantitative trait locus (QTL) gene t contributes to the phenotypic variation in a trait? I. DNA and protein sequence analyses are used to determine if there is a correlation with phenoty variation
II. Each of the offspring is individually backcrossed with the same parent, usually the one expressi phenotype more strongly
III. Lod scores are calculated for each genetic marker to find QTLs
IV. The genotype of the progeny at genetic markers located every 5- 10 cM is determined for each introgression line
V. Two individuals which exhibit the extremes of a specific phenotype are crossed
VI. DNA sequence variants among introgression lines are analyzed to find candidate genes

A) V, II, IV, III, VI, I
B) V, IV, III, II, VI, I
C) V, IV, II, III, I, VI
D) I, V, III, II, IV, VI
E) I, II, IV, III, VI, V