A variety of E. coli clones have been isolated, the DNA has been extracted, digested with restriction enzymes, denatured, separated by gel electrophoresis, and transferred to nylon membrane by the Southern blot procedure. A radioactive probe is used that hybridizes with a gene of interest. The DNA corresponding to a single clone yields a single DNA band after probing the blot. Based on this information only,
A)the gene of interest is located entirely on the DNA band visualized.
B)only the one clone described has the gene of interest.
C)all of the clones tested, except the one described, have the gene of interest.
D)the part of the gene recognized by the probe is located on the DNA band visualized.
E)both B and D

Question

Quizplus · Accepted Answer

The Answer of A variety of E. coli clones have been isolated, the DNA has been extracted, digested with restriction enzymes, denatured, separated by gel electrophoresis, and transferred to nylon membrane by the Southern blot procedure. A radioactive probe is used that hybridizes with a gene of interest. The DNA corresponding to a single clone yields a single DNA band after probing the blot. Based on this information only,
A)the gene of interest is located entirely on the DNA band visualized.
B)only the one clone described has the gene of interest.
C)all of the clones tested, except the one described, have the gene of interest.
D)the part of the gene recognized by the probe is located on the DNA band visualized.
E)both B and D

A Variety of E