You are designing, by genetic engineering, a protein that needs to be synthesized in the cytoplasm and then localized in the nucleus of the cell. To accomplish this one could add
A)the compound during mitosis, at a time when the nuclear membrane is dispersed.
B)a series of specific polysaccharides to the carboxyterminal end of the protein.
C)a nucleotide or few nucleotides to the protein.
D)a nuclear localization signal into the DNA that will be present in the mature protein.
E)all of the above

Question

Quizplus · Accepted Answer

A nuclear localization signal (NLS) is a sequence of amino acids that 'tags' a protein for import into the nucleus by nuclear transport. Adding an NLS to the DNA ensures it will be present in the mature protein, directing it to the nucleus.

You Are Designing, by Genetic Engineering, a Protein That Needs