Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of ________.
A) post-transcriptional processing
B) post-translational processing
C) nucleic acid hybridization
D) restriction fragment ligation

Question

Quizplus · Accepted Answer

The use of mRNA and reverse transcriptase in cloning eukaryotic genes into bacterial cells addresses the challenge of post-transcriptional processing. Eukaryotic mRNA, which has already undergone splicing to remove introns, is reverse-transcribed into cDNA, bypassing the need for the bacterial host to perform eukaryotic-like post-transcriptional modifications.

Expression of a Cloned Eukaryotic Gene in a Bacterial Cell