Indicate whether each of the following manipulations or procedures used in current recombinant DNA technology commonly rely on an enzyme (E) or are done nonenzymatically (N). Your answer would be a six-letter string composed of letters E and N only, e.g. EEEEEE.
( ) Cleavage of DNA at specific sites
( ) DNA ligation
( ) DNA cloning
( ) Nucleic acid hybridization
( ) Synthesis of DNA of any desired specific sequence
( ) DNA sequencing

Question

Indicate whether each of the following manipulations or procedures used in current recombinant DNA technology commonly rely on an enzyme (E) or are done nonenzymatically (N). Your answer would be a six-letter string composed of letters E and N only, e.g. EEEEEE.
( )	Cleavage of DNA at specific sites
( )	DNA ligation
( )	DNA cloning
( )	Nucleic acid hybridization
( )	Synthesis of DNA of any desired specific sequence
( )	DNA sequencing

Quizplus · Accepted Answer

Hybridization is mostly done nonenzymatically, as is chemical DNA synthesis. Current major DNA sequencing methods rely on enzymes (e.g. polymerases), but some nonenzymatic methods are also in development.

Indicate Whether Each of the Following Manipulations or Procedures Used