RNA-seq and ribosome profiling experiments have been carried out on the same cells. The following simplified graphs show the results for the same genomic region containing a gene with three exons. Which graph (1 or 2) do you think corresponds to the RNA-seq results? What feature is represented by the arrow?
A) Graph 1; stop codon
B) Graph 1; polyadenylation site
C) Graph 2; stop codon
D) Graph 2; polyadenylation site

Question

RNA-seq and ribosome profiling experiments have been carried out on the same cells. The following simplified graphs show the results for the same genomic region containing a gene with three exons. Which graph (1 or 2) do you think corresponds to the RNA-seq results? What feature is represented by the arrow? 
A) Graph 1; stop codon 
B) Graph 1; polyadenylation site 
C) Graph 2; stop codon 
D) Graph 2; polyadenylation site

Quizplus · Accepted Answer

Peaks in the RNA-seq result also cover the 5′ and 3′ untranslated regions of the mRNAs.

RNA-Seq and Ribosome Profiling Experiments Have Been Carried Out on the Same