Sort the following steps in the common procedure to create transgenic plants. Your answer would be a four-letter string composed of letters A to D, e.g. DABC.
(A) Callus growth
(B) Agrobacterium infection
(C) Shoot/root induction
(D) Removal of leaf tissue from a plant

Question

Sort the following steps in the common procedure to create transgenic plants. Your answer would be a four-letter string composed of letters A to D, e.g. DABC.
(A)	Callus growth
(B)	Agrobacterium infection
(C)	Shoot/root induction
(D)	Removal of leaf tissue from a plant

Quizplus · Accepted Answer

Transgenic plants can be created from plant cells transfected with DNA in culture. Normally, a disc is cut out of a leaf and incubated in a culture of Agrobacterium that carries the desired gene as well as a selectable marker on a plasmid. Only cells transfected with the plasmid are then able to proliferate and form a callus on the selection medium. With careful manipulation of the nutrients and growth regulators, a whole new plant can then be generated from the callus.

Sort the Following Steps in the Common Procedure to Create