Solved

From an Immortalized Human HeLa Cell Line with Wild-Type P53

Question 34

Short Answer

From an immortalized human HeLa cell line with wild-type p53 genes, you have derived a line that lacks both copies of the gene. You then treat the original and derived cells with the anticancer drug doxorubicin, which can activate the p53 pathway in the cell by stalling DNA replication forks and inducing double-strand breaks in DNA. You measure cell proliferation in the presence of different doses of the drug in each of the two cell lines, and plot the results as shown in the graph below. Which cell line (A or B) do you expect to be the original HeLa line? Write down A or B as your answer. From an immortalized human HeLa cell line with wild-type p53 genes, you have derived a line that lacks both copies of the gene. You then treat the original and derived cells with the anticancer drug doxorubicin, which can activate the p53 pathway in the cell by stalling DNA replication forks and inducing double-strand breaks in DNA. You measure cell proliferation in the presence of different doses of the drug in each of the two cell lines, and plot the results as shown in the graph below. Which cell line (A or B) do you expect to be the original HeLa line? Write down A or B as your answer.

Correct Answer:

verifed

Verified

Cells in the original HeLa li...

View Answer

Unlock this answer now
Get Access to more Verified Answers free of charge

Related Questions

Unlock this Answer For Free Now!

View this answer and more for free by performing one of the following actions

qr-code

Scan the QR code to install the App and get 2 free unlocks

upload documents

Unlock quizzes for free by uploading documents